Amplification of Specific Genes by using RT-PCR Technique in Plants

Rapid Detection of COVID-19 by RT-LAMP PCR Technique and its Comparison with Real-Time RT-PCR Method

Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used for the detection of microorganisms worldwide. Objectives: This study aimed by Isothermal nucleic acid amplification techniques to detect the covid-19. Methods: In this study, 200 samples of nasopharynx and oropharynx were collected from Jamaran Heart Hospital in Tehran. Covid -19 was exami...

Field Study of Infectious Bronchitis Virus in Broiler Using Type-Specific RT-PCR

Serotype Identification of Recent Iranian Isolates of Infectious Bronchitis Virus by Type-Specific Multiplex RT-PCR

PCR Typing of Trichophyton Rubrum Isolates by Specific Amplification of Subrepeat Elements in Ribosomal DNA Nontranscribed Spacer

Background: Trichophyton rubrum (T. rubrum) is the most common cause of dermatophytosis of skin and nail tissue. Strain identification in Trichophyton rubrum is important for identification of strain-related differences in infectivity potential or transmissibility and epidemiological studies. PCR typing could determine whether the original isolate is responsible for re-infection or a new strain...

Amplification of four genes of influenza A viruses using a degenerate primer set in a one step RT-PCR method.

We designed a degenerate primer set that yielded full-length amplification of hemagglutinin (HA), neuraminidase (NA), matrix (M), and non-structural protein (NSP) genes of influenza A viruses in a single reaction mixture. These four genes were amplified from 15 HA (1-15) and 9 NA (1-9) subtypes of influenza A viruses of avian (n=16) origin. In addition, 272 field isolates of avian origin were t...